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KMID : 0351619740150020165
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Kyungpook Medical Journal
1974 Volume.15 No. 2 p.165 ~ p.175
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Effect of Reduced Glutathione on the Contednts of Protein and Sulfhydryl groups of the Liver and Lung Tissues of the Rabbit Following Carbon Tetrachloride Intoxication
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Abstract
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The present study was undertaken to clarify the hepatotoxic effect of carbon tetrachloride(CCl₄) and to observe a possible toxic effect of CCl₄ on the lung tissue, as well as to elucidate the effectiveness of reduced glutathione(GSH) to the CCl₄ intoxicated animal.
The rabbit was used throughout the entire experiment, and the mixture of CCl₄ and olive oil (1 : 1) in dose of 0.3㎖ or 0.5㎖ per kg BW were administered intraperitoneally twice weekly for one or two weeks, and changes of protein, NP-SH (non-protein sulfhydryl) and NP-SS (non-protein disulfide) of the liver as well as body weight change were observed one hour after the respective CCl₄ administration as a preliminary experiment to determine the appropriate dose of CCl₄ in the main experiment.
All the experimental data obtained from the preliminary study showed decreased values from the control, but when the CCl₄ mixture of 0.3㎖ per kg BW was administered twice weekly for one week te decrease was not as prominent as when higher doses of CCl₄ were used, in which cases the decrease was similar.
From the above results, the CCl₄ mixture of 0.3㎖ per kg BW was used twice weekly for two weeks in the main experiment, and the changes of protein, NP-SH and NP-SS of the liver and lung tissues were studied on 1,3 and 5 days after CCl₄ administration. The changes of body weight and liver weight were also measured, and the liver weight/body weight ratios were thus calculated.
The results are summarized as follows:
1. The body weight of the control group was 2.23±0.17Kg and the contents of protein. NP-SH and NP-SS of the liver was 102.31±15.30 mg/gm wet wt, 8.01±0.81μmol/gm wet wt and ??.33±0.52 μmol/gm wet wt, respectively. The contents of protein, NP-SH and NP-SS of the lung in the control group was 47.35±9.85 mg/gm wet wt, 3.01±0.4μmol/gm wet wt and 0.38±0.48μmol/gm wet wt, respectively.
2. When 0.3㎖ of the mixture of CCl₄ and olive oil per kg BW was administered twice weekly for two weeks, the contents of protein and sulfhydryl groups of the liver decreased noticeably from the control values, while the administeration of GSH caused no change from the control. When GSH and CCl₄ were combined, the values were lower than GSH group, but higher than CCl₄ group.
3. The contents of protein and sulfhydryl groups in the lung showed decreased values from the control when CCl₄ was administered, but no change was observed when GSH was administered, and when GSH and CCl₄ were combined the values showed intermedially between the CCl₄ and GSH groups.
4. The decrease of the body weight by CCl₄ injection was prominent but the liver weight increased noticeably. Thus, the liver weight/body weight ratios were elevated from the control value of 20.27 throughout the experiment. When GSH was used, the ratio did not change from the control but the combined administeration of GSH and CCl₄ produced higher ratio than the CCl₄ group.
5. From the above, it may be concluded that CCl₄ produces a toxic effect to the lung as well as to the liver, but the degree of toxicity seems to be less in the lung than in the liver. Also, the effect of GSH seems to be not as obvious in the lung as in the liver.
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